There will be 43 civilizations in Civ5, each with its own leader and unique unit. In addition, every leader has a semi-unique "personality", consisting of traits that determine their in-game behavior.

For example, Elizabeth of the English, a historical naval power, emphasizes a pursuit of naval dominance and the choices she makes reflects this desire. However, in order to prevent leaders from becoming too-predictable, these traits have the potential to be changed slightly and weighted differently before a new game begins, keeping diplomacy fresh.

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There are eight playable leaders in Colonization: two each for the English, French, Dutch, and Spanish colonies. Each leader has two traits, or characteristics that yield a special bonus. One of the traits is unique to that leader, and the other is shared by both leaders of a particular nation. For example, George Washington of the English colonies is Tolerant and Disciplined. John Adams, the other English leader, is also Tolerant, but instead of being Disciplined, he is Libertarian, a trait that no other leader has.

In addition to the leaders for European colonies, there are a few others: chiefs of native tribes and European kings. In total, there are eight different native tribes which inhabit the New World, each with their own leader and two traits, and four Kings, one for each European country.

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